Category Archives: Data file recovery

Finding journal articles – information technology – library guides at monash university

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Pesticide safety training and train-the-trainer programs

December 2016: Letter to all current Train-the-Trainer Instructors about complying with the Worker Protection Standard and Interim US EPA Approval numbers for 2017, PDF (148 kb) Background A written pesticide safety training program is required for training employees who handle

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WASHINGTON — Hardening battle lines for the brawl to come, President Barack Obama urged congressional Democrats to “look out for the American people” in defending his legacy health care overhaul, while Vice President-elect Mike Pence stood firm Wednesday in telling

A novel genetic variant database for korean native cattle_文库下载

16etal.2012).Despitetheirbriefhistory,Hanwooisnowregardedasapremiumbeefandanationalfavorite,despiteitsrelativehighprice,andthebreedhasbeenrelativelywell-maintained.Hanwoo,now,havesubstantialcom-mercialvalueintheKoreanlivestockindustry(Hwangetal.2010). Inthelivestockindustry,geneticresearchhascontrib-utedtooptimizationofbreeding,productivity,andproductquality.Furthermore,theimportanceandutilityofgeneticshavebeenincreasingduetoimprovedtechnologiesanddataanalysestechniques.SinceHanwoohavemaintainedstabletraitsthroughpurebreeding,thecurrentbloodlineageisvaluableandspreadoutmainlyintheKoreanPeninsula(KimandLee2000).Additionally,HanwoooccupyasubstantialproportionofthelivestockindustryinKoreamakingthegeneticinformationimportantfortheKoreanlivestockindustry. Inthecaseofcattle(BosTaurus),awholegenomedata-baseisalreadyinexistence;theBovineGenomeDatabase(BGD,http://www.wenkuxiazai.com)isagenomeinformaticsresourceforBosTauruswhichprovidesdatathroughgen-omebrowsers,BLASTsearching,andgenequeryinterfaces.Italsodisplaysgenepredictions,non-codingRNA,repeats,pseudogenes,SNPs,markers,QTL,andalignmentsofpro-teinhomologsandtranscriptsequences(Reeseetal.2010).Moreover,aSNPannotationtoolforcattlealsoexists,andthistoolprovidesannotationinformationforSNPsbyinte-gratingvarioussourcesofgenomicinformationsuchasEntrezGeneandUniProt(Jiangetal.2011). Geneticspeci cationofHanwootraitsisneededtomain-tainthebreed.ResearchonthegeneticpropertiesofHanwoohasbeenconductedbyvariousgroupsandfragmentsofinformationforgeneticvariantsofHanwoohavebeenpro-duced.Forexample,anumberofstudiesonexpressedgenesrelatedtomeatqualityofHanwoohavebeenconducted.Researchershaveidenti ed14candidategenesrelatedtointramuscularfatdevelopment(Leeetal.2007)andanalyzed11candidategenesrelatedtomarblinginHanwoobyRT-PCR(Leeetal.2008a).Geneticvariants(SNPs)ofHanwoohavebeenstudiedaswell.ApreviousstudyattemptedtoidentifySNPmarkerstoestimatemarblingscoreandmeatqualitygradeforHanwoo(Shinetal.2012).Thisstudyrevealedthatfattyacidbindingprotein4(FABP4)geneknowntoberelatedtointramuscularfathasthreeSNPsandoneofthosewasrelatedtomarblingscoreandmeatqualityforHanwoo.Additionally,44SNPsrelatedtocarcassqualitytraitswereidenti edusingDNAchipsinHanwoopopulations(Leeetal.2010).Inaddition,todetecttheoriginofbeef,astudywasconductedtoidentifySNPmarkersthatcharacterizetheHanwoobreed.Inthisstudy,researchersidenti ed90SNPmarkersthatcharacterizeHanwoobreed(Cheongetal.2013). AlthoughmanystudiesongeneticinformationofHanwoohavebeenconducted,awholegenomedatabaseofHanwooproducedbyNextGenerationSequencing(NGS)technologythatofferseasyaccesstogeneticinformationfortheresearchersandbreedershasyettobedeveloped. HanwooGDBprovidesSNPdataforthewholegenomeofHanwooproducedbyNGStechnology.SincethisdatabasealsoprovidesSNPchipdataofreferencesequences,itcanbeusedtochecktheconcordanceofpositionofeachSNPbyNGStechnology.Itprovidesanumberofstatisticsrelatedtodataproductionandvalida-tion,whichcanbeaccessedbyallusers,aswellasincludesannotationinformationofgenesforeachSNPandSNPdataforeachindividual.Thus,itcouldprovideinferencetohelpunderstandthefunctionalaspectsofthesevariantsandthespeci ccharactersofHanwoobreed.Almostalloftheinformationinthisdatabasecanbedownloadedinvcfformat,whichiseasilyapplicabletootherdataforbothresearchersandbreeders.Moreover,thisdatabaseprovidesbrowserstohelpunderstandthecomprehensiveinforma-tionofthesevariantsandvisualizethedatabase.ThisdatabasewillcontributetogeneticresearchesandbreedingstrategiesofHanwoo. Bloodsampleswerecollectedfrom12HanwoocattlefromKyungpookNationalUniversity,Korea,and11HanwoocattlefromtheNationalInstituteofAnimalScience,Korea.Samples(10ml)weredrawnfromthecarotidarteryandtreatedwithheparintopreventclotting.GenomicDNAsampleswereextractedfrombloodsamplesusingaG-DEXTMIIbGenomicDNAExtractionKit(iNtRoNBiotechnology,Seoul,Korea).Randomlysheared3lgofgenomicDNAwasusedtogenerateDNAfragmentsabout300bpbytheCovarisSystem.ThefragmentsofshearedDNAwerepassedthroughpreparationprocessesforsequencingincludingend-repaired,A-tailed,adaptorliga-ted,andampli edusingaTruSeqDNASamplePrep.Kit(Illumina,SanDiego,CA).Forpreparedsamples,paired-endsequencingwasconductedbyNationalInstrumentationCenterforEnvironmentalManagement(NICEM),Seoul,Korea,usingtheIlluminaHiSeq2000platformwithTru-SeqSBSKitv3-HS(Illumina).Here,weperformedsequencingononelaneperonesample.Asaresultofthesequencingprocess,weacquired42,534,069,646paired-endreadsintheaverageof23Hanwoocattleandthemeancoverageratiowas15.93.

Migrating databases from sql server 2000 to sql server 2012 using powershell – stuart moore

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Identify servers and the databases mounted in sharepoint – sqlservercentral

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Eventbrite _ will jangmadang (markets) lead to an eventual opening of north korea_

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